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The Journal of Biological Chemistry
Year: 2009  |  Volume: 284  |  Issue: 23  |  Page No.: 15598 - 15606

Photosystem II Complex in Vivo Is a Monomer

T Takahashi, N Inoue Kashino, S. i Ozawa, Y Takahashi, Y Kashino and K. Satoh    

Abstract:

Photosystem II (PS II) complexes are membrane protein complexes that are composed of >20 distinct subunit proteins. Similar to many other membrane protein complexes, two PS II complexes are believed to form a homo-dimer whose molecular mass is ~650 kDa. Contrary to this well known concept, we propose that the functional form of PS II in vivo is a monomer, based on the following observations. Deprivation of lipids caused the conversion of PS II from a monomeric form to a dimeric form. Only a monomeric PS II was detected in solubilized cyanobacterial and red algal thylakoids using blue-native polyacrylamide gel electrophoresis. Furthermore, energy transfer between PS II units, which was observed in the purified dimeric PS II, was not detected in vivo. Our proposal will lead to a re-evaluation of many crystallographic models of membrane protein complexes in terms of their oligomerization status.

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