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The Journal of Biochemistry
Year: 2010  |  Volume: 147  |  Issue: 1  |  Page No.: 63 - 71

A lentiviral vector encoding two fluorescent proteins enables imaging of adenoviral infection via adenovirus-encoded miRNAs in single living cells

Y Kato, S. Y Sawata and A. Inoue    

Abstract:

MicroRNAs (miRNAs) are non-coding small RNAs that have been found in various kinds of eukaryotes and viruses. Recently, adenovirus non-coding RNAs, VA RNAs, have been reported to generate miRNAs. Here, we developed a lentiviral vector for monitoring adenovirus-derived miRNAs in living cells. By using red and green fluorescent proteins under the control of bi-directional two distinct promoters, adenoviral infection and consequent miRNA expression was successfully visualized and quantified by the reduction in green fluorescence when 3'-untranslated regions were connected to the target sequences of the adenovirus-derived miRNAs. Our functional analysis using a lentiviral vector is a useful method to examine the activity of miRNA in living mammalian cells.

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