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Journal of Animal and Veterinary Advances
Year: 2015  |  Volume: 14  |  Issue: 13  |  Page No.: 393 - 398

Molecular Cloning, Characterization and Tissue Distribution of MyoGenin Genein Goose (Anser cygnoides)

Cui Wang, Yi Liu, Yunzhou Yang, Huiying Wang, Sifeng Yi, Chuang Li, Shaoming Gong, Weihu Chen and Daqian He    

Abstract: MyoGenin (MyoG) is a basic Helix-Loop-Helix (bHLH) transcription factor that belongs to the Muscle-specific transcription Factors (MRFs) family which plays critical roles in regulating the skeletal muscle development and growth. In this study, the complete coding sequence and genomic DNA sequence of goose MyoG gene were cloned and characterized. The goose MyoG CDS was composed of 684bp that encoded a 227 amino acid protein, including a highly conserved basic helix-loop-helix domain. Multiple sequence alignments and phylogenetic analysis indicated that the deduced goose MyoG protein was conserved in vertebrates, especially in the avian species. The goose MyoG genomic DNA sequence we obtained was 3444bp and consisted of 3 exons and 2 introns.Sem-quantitative RT-PCR analysis demonstrated that the goose MyoG mRNA was specifically expressed in the breast muscle and leg muscle tissues, little or no expression was observed in heart, liver, spleen, lung, kidney, muscular stomach, brain, intestine and sebum. These data will serve as a foundation for further insight into the functions of the MyoG gene in Chinese domestic goose.

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