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Journal of Animal and Veterinary Advances
Year: 2015  |  Volume: 14  |  Issue: 1  |  Page No.: 13 - 17

Biochemical Differentiation Between Hyla orientalis (Bedriaga, 1890) and Hyla savignyi (Audoin, 1827) as Revealed by the Skeletal Muscle Protein Bands

Ali Ihsan Eroglu and Ufuk Bulbul    

Abstract: In this study, in order to investigate biochemical differences among skeletal muscle protein bands of the Hyla orientalis specimens from three populations and H. savignyi specimens from six populations in Turkey were compared by SDS-PAGE (Sodium Dodecyl Sulphate-Poly Acrylamide Gel Electrophoresis). Totally, 26 adult (14 females and 12 males) H. orientalis specimens and 54 adult (25 females and 29 males) H. savignyi specimens were used in SDS-PAGE characterization. The first group (Bursa, Edirne and Trabzon populations of H. orientalis) had 34 homologous protein bands whereas the second group (Adana, Hatay, Gaziantep, Kilis, Siirt and Elazig populations of H. savignyi) had 32 bands. It was shown that the first group had two additional and unique bands. The results of the present study show that specimens of the H. orientalis and H. savignyi populations in Turkey are biochemically different according to total number of skeletal muscle protein bands.

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