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Journal of Animal and Veterinary Advances
Year: 2014  |  Volume: 13  |  Issue: 10  |  Page No.: 612 - 615

The Evaluation of Ascorbic Acid on Growth and Proteolysis Activity of Pseudomonas fluoresencens Isolates from Refrigerate Raw Milk

Behira Belkacem    

Abstract: Psychrotrophic Gram-negative bacteria such as Pseudomonas species, pose a significant spoilage problem in refrigerated raw milk and dairy products due to secretion of hydrolytic enzymes, especially lipases and proteases. This study evaluated the effect of ascorbic acid against proteolysis activity produced by Pseudomonas fluoresencens in refrigerate raw milk. Strains of Pseudomonas fluorescens isolated from raw milk. Samples of pasteurized milk were inoculated with these strains and stored 10 days at 7°C. All the isolates showed proteolysis activity. Different concentrations of ascorbic acid were tested and the protein content was determined. The growth of each isolate tested was decreased and the content of protein was also decreased in accordance with the bacterial count.

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