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Journal of Animal and Veterinary Advances
Year: 2014  |  Volume: 13  |  Issue: 5  |  Page No.: 274 - 279

A Modified CBBR-250 Microwave-Assisted Staining and Destaining Method on SDS-PAGE Gels

Yun-Tao Ji, De-Yu Chen, Shuang-Lin Jiang and Chang-Qing Qu    

Abstract: The application of microwave-assisted technologies has drastically reduced overall staining and destaining times in CBB R-250 staining on SDS-polyacrylamide gels but accurate temperature control and reproducibility for conventional commercial microwaves are not satisfactory. The objective of this study is to improve the existing procedures and develop an optimal method for using microwave-assisted technologies. Researchers adjusted the component of staining and destaining solutions and attempted to control the microwave temperature by shortening time, the staining and destaining could be completed within 30 min and resulted in dark purple-blue protein bands. In addition, the SDS-polyacrylamide gel displayed a transparent gel background. The detection limit for bovine serum albumin and fetuin-A on the SDS-polyacrylamide gels was 0.01 μg. These results showed that the modified method required less time. Furthermore, the modified procedure minimized the use of harmful organic solvents due to ethanol instead of methanol, methanol is more toxic than ethanol yet it maintained a high sensitivity for protein staining on SDS-polyacrylamide gels.

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