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Journal of Animal and Veterinary Advances

Year: 2013  |  Volume: 12  |  Issue: 7  |  Page No.: 823 - 830

Establishment and Characterization of a Fibroblast Line from Inner Mongolia Cashmere Goats

Peng Cui, Pengfei Hu, Xiangchen Li, Weijun Guan and Yuehui Ma

Abstract

A fibroblast line (named SCF36) from 33 Inner Mongolia Cashmere goats ear marginal tissues was established successfully by means of using primary explant technique and cell cryoconservation technology. This fibroblast line contains 336 cryovials with 8x106 cells, respectively. Biological characteristics of the fibroblast line showed that the cells cultured in vitro were all morphologically typical fibroblast and the cell Population Doubling Time (PDT) was approximately 2 days. The cell average viability before freezing was 97.37±2.25 and 91.43±.22% after thawing. Chromosome analysis showed that >90% of the whole population were diploid. Isozyme analysis of Lactic Dehydrogenase (LDH) and Malic Dehydrogenase (MDH) showed that the SCF36 cells had no cross-contamination with other species and the genetic characteristics of the cell line were stable in vitro. Tests for cell line contamination with bacteria, fungi and mycoplasmas were also negative. The transfection efficiency of three fluorescent proteins were relatively high, indicating that the exogenous genes could be effectively expressed in the cells. The cell line met all criteria from the American Type Culture Collection (ATCC). Not only has the germline of this important sheep breed been preserved at the cell level but also valuable material had been provided for genome, postgenome and somatic cloning research and so on.

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