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Journal of Animal and Veterinary Advances
Year: 2013  |  Volume: 12  |  Issue: 6  |  Page No.: 788 - 790

A Novel PCR-RFLP (Xba I) Polymorphism Analysis of the Bovine Calpastatin (CAST) Gene in Chinese Luxi Cattle

Dongying Yang    

Abstract: Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases-μ-calpain and m-calpain, found in mammalian tissues. In the coding region of the bovine CAST gene (CAST) the new nucleotide sequence polymorphism was found being a substitution G→C at position 962 nt (consensus sequence-GenBank AF281256). Consensus of bovine CAST sequence with that of human revealed that G→C substitution was located at position 1460 nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution for CAST protein. The frequency of alleles C and G was 0.41 and 0.59, respectively. The message from these results is that CAST gene may probably be considered as a candidate marker for beef quality.

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