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Journal of Animal and Veterinary Advances
Year: 2013  |  Volume: 12  |  Issue: 20  |  Page No.: 1530 - 1536

Establishment and Biological Characteristic Research of Altay Sheep Fibroblast Cell Bank

Hui Wang, Xiaohong He, Taofeng Lu, Yuhua Zhao, Zhiqiang Zhu, Weijun Guan and Yuehui Ma    

Abstract: A fibroblast bank of Altay sheep consisting of 182 tubes of frozen cells was successfully abtained from primary explants of 57 individual animals through attachment cultivation and cryopreservation biotechniques and each vial contains 1.3x107 cells, respectively. The results of quality assays and biological characteristic researches showed that the cells cultured with a Population Doubling Time (PDT) of about 24 h, constituted a typical fibroblast in morphology; assays for microbial contamination regarding bacteria, fungi, viruses and mycoplasmas are all negative; analysis of Lactate Dehydrogenase (LDH) and Malate Dehydrogenase (MDH) isoenzymes ruled out the possibilities of cross-contamination between species; the ratio of diploid cells is 98.6%. In order to study the expressibility of exogenous genes, six kinds of fluorescent protein-coding plasmids were transfected into the Altay sheep fibroblast with transfection efficiencies between 22.3 and 46.7%. This research could not only preserve the genetic resources of Altay sheep but also provide both technical and theoretical basis for preserving those of other mammals and poultry at cell level.

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