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Journal of Animal and Veterinary Advances
Year: 2013  |  Volume: 12  |  Issue: 13  |  Page No.: 1183 - 1189

Serotype Distribution and Detection of Enterotoxin Gene of Chicken Source of Salmonella in the Eastern Part of Hebei Province

Qiumei Shi, Yanying Zhang, QiuYue Wang, Guisheng Gao, Guangping Gao, Hai Fang, Donglin Zhang, Cuizhen Chen and Xiaomei Lv    

Abstract: To explore the pathogenic mechanism of Salmonella, serotype was identified and enterotoxin gene stn was detected for 47 strains suspected Salmonella in the Eastern part of Hebei Province. According to the bacterial culture characteristic, physicochemical properties and analysis results of K-3401 semi automatic bacteria identification instrument and identification for another time by Chengdu Institute of Biological Products, enterotoxin gene stn was detected by PCR. The 17 strains of Salmonella gallinarum, 14 strains of Salmonella typhimurium, 4 strains of Salmonella pullorum 2 strains of Salmonella paratyphi A, 2 strains of Salmonella group BO, 5 strains of Salmonella bovismorbificans, 3 strains of Salmonella enteritidis were detected from 47 strains of chicken source of Salmonella. The 45 strains of stn gene were amplified successfully in all 47 strains. The carrying rate was 95.7%. Homology of stn gene of test strains of Salmonella was between 94 and 100%. Evolutionary tree display that different serotypes of Salmonella enterotoxin stn gene divided into 5 groups. There was no homology with other bacterium. Chicken source of Salmonella contained 7 kinds of serotypes in the eastern part of Hebei Province. Among them, Salmonella gallinarum was 36.2% (17/47), Salmonella typhimurium which was advantages serotypes was 29.8% (14/47). The carrying rate of enterotoxin gene stn which had relation with bacterial pathogenicity was 95.7% (45/47).

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