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Journal of Animal and Veterinary Advances
Year: 2013  |  Volume: 12  |  Issue: 11  |  Page No.: 1021 - 1031

The Culture of Embryonic Stem Cells Derived from in vivo Fertilized Embryos of Arbas Cashmere Goats

Muzi Jin, Hui Wu, Wenliang Yang, Fei Hao, Dapeng Tai, Jianlong Yuan, Asga, Ming Cang, Xudong Guo and Dongjun Liu    

Abstract: Research on the embryonic stem cells of large livestock has recently attracted the attention of scholars. However, it is difficult to keep goat embryonic stem cells in an undifferentiated state during cell passaging in culture. In this study, fertilized embryos of Arbas cashmere goats were obtained by superovulation in vivo. The key issues in the culture of Arbas cashmere Goat Embryonic Stem Cells (AgESCs) were explored: the addition of differentiation-inhibiting factors, the selection of medium as well as the passaging, cryopreservation and thawing methods used. This study found that high-quality in vivo fertilized embryos could be cultured in either serum-containing medium or serum-free medium and that AgESCs could be passaged in either medium for 30 generations. The mechanical method was superior to the trypsin digestion method for passaging AgESCs. There was no change before and after cryopreservation and thawing with regard to AgESC morphology, alkaline phosphatase staining, the formation of embryoid bodies, immunofluorescence staining or the PCR detection of pluripotency factors. Finally this study provides the experimental basis for the establishment of goat embryonic stem cell lines.

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