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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 4  |  Page No.: 483 - 487

The Use of Chicken Embryos as a Model for Brucella abortus Strains Isolation from Milk Collected Samples

C.E. Pulido, H.D.I. Martinez, V.M. Villanueva, O.V. Amador, C.A. Peniche, S.V.T. Pardio, G.L.A. Landin, C.R. Flores, A.J.F. Morales and M.A. Lopez    

Abstract: A total 45 conventional Chicken Embryos (CE), 3-7 days old were divided in three groups of 15 to evaluate CE as an infection model to increase Brucella abortus concentration from milk samples. Group I was inoculated with RB51 strain, group II with S19 and group III was used as a not inoculated control. About 100-120 Colony Forming Units (CFU) of microorganisms/0.5 mL of ultra pasteurized milk were inoculated into the yolk sac. All groups were incubated for 72 h at 37°C and then slaughtered to collect liver, spleen, kidney and bone marrow. Organs were inoculated by duplicate on Tripticasein Soy Agar culture medium (TSA) and half were incubated under aerobic conditions and the other half with 5-10% CO2 environment during 8 days. The results obtained were analyzed using Chi-square. After incubation, 9/15 (60%) CE from group I, 11/15 (73.3%) from group III and 13/15 (86.7%) from group III were alive, respectively. Inoculated groups did not show any significant differences with the control (p>0.05). Isolated concentrations in organs were higher than 100,000 CFU g-1 of RB51 strain from 6/9 CE of group I and S19 strain from 7/11 of group II while in group III, all Brucella sp. strains were isolated. RB51 strain was harvested from 5/6 livers, 2/6 bone marrows, 1/6 spleens and 1/6 single kidney. S19 strain was harvested from 3/7 livers, 2/7 spleens and 1/7 single kidney. It was concluded that the CE model is a suitable alternative to increase Brucella abortus concentration from infected milk.

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