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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 23  |  Page No.: 4369 - 4373

Cloning and Expression of Chicken AvBD8 Mature Peptide Gene in Lactococcus lactis

Kezong Qi, Liu Miao, Kaisong Peng, Jian Tu and Xiuheng Xue    

Abstract: Chicken Beta Defensin-8 (AvBD8) is an antibacterial peptide that plays significant roles in innate immunity. The aim of this study was to investigate the expression of Chicken avian AvBD8 mature peptide gene in Lactococcus lactis (L. lactis) system. In this study, the AvBD8 gene was cloned and sequenced from chicken liver by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Then, the AvBD8 mature peptide gene was amplified and cloned into the expression vector pNZ8048. The result showed that the recombinant vector pNZ8048-AvBD8 that verified by colony PCR, double digestion and DNA sequencing was transformed into the competent cell L. lactis NZ9000 by electroporation. The expression of the recombinant protein AvBD8 was confirmed by tricine SDS-PAGE analysis. The present findings suggest that L. lactis could act as a host for the production of avian beta defensins for further study.

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