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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 20  |  Page No.: 3849 - 3854

Characterization of β-Actin Promoter of Leuciscus merzbacheri

Wenge Hu, Shiwei Ma, Chuangfu Chen, Yuanzhi Wang, Yan Ren, Xudong Cao and Jinliang Sheng    

Abstract: Leuciscus merzbacheri is a unique vulnerable indigenous fish which is only distributed in the Junggar Basin, Xinjiang. In this study, researchers cloned two L. merzbacheri β-actin promoter fragments of different length: SZ11 and SZ21 and analyzed their structural features. The mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression vectors β1 pEGFP-N1-AFP III and β2 pEGFP-N1-AFP III in which fish type III antifreeze protein gene was used as the structural gene. The results showed that the cloned two fragments of β-actin promoters had the ability to drive the expression of the green fluorescent protein gene in BHK-21 cells. These data suggest that the vectors researchers constructed based on β-actin promoter could be exploited as all fish recombinant eukaryotic expression vector.

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