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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 16  |  Page No.: 3027 - 3030

The Expression Analysis of Ghrelin and Growth Hormone Secretagogue Receptor in Mouse Thymic Epithelial Cells

Jian-Hong Liu, Yong-Jiang Ma, Ying Li, Hai-Bin Peng, Dao-Tong Li, Yuan Zhan and Yu-Gu Li    

Abstract: The present study was to identified the expression of Ghrelin and GHS-R in mouse Medullary-Type Epithelial Cells (MTEC1). Whether the gene of ghrelin and GHS-R are expressing in MTEC1 cells or not was measured by using Quantitative real time RT-PCR (Q-PCR). Results identified that the mRNA of ghrelin and GHS-R can be detected in MTEC1 cells. The protein levels of GHS-R and ghrelin precursor were detected by the method of Western blot analysis. Moreover, the location of GHS-R in MTEC1 cells were detected by immunofluorescence and the quantity of ghrelin in the supernatant of MTEC1 cells were measured by ELISA. The results of Western blot analysis make sure that GHS-R and precursor of ghrelin are expressing in MTEC1 cells. GHS-R is a kind of transmembranes protein. As the results of immunofluorescence shown the quantity of ghrelin in the supernatant of MTEC1 cells measured by ELISA was 39.20 pg mL-1 while the control was 5.74 pg mL-1.

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