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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 14  |  Page No.: 2588 - 2593

Inflammatory Response of Peripheral Blood Mononuclear Cells Post Intramammary Challenge with Staphylococcus aureus in Dairy Cows

Li Liu, Zhengrong Yuan, Cui Chen, Limin Zhang, Xiaojie Chen, Xidong Liu, Xue Gao, Junya Li, Huijiang Gao, Lupei Zhang and Shangzhong Xu    

Abstract: To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of Staphylococcus aureus (S. aureus) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX3C Chemokine Receptor 1 (CX3CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with S. aureus relative to uninfected controls (n = 3) were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of IL-8, CD14 and CCR2 gene were exhibited significantly (p<0.05) up-regulated within 2 dpi while there was no significant difference in the expression of M-CSFR, TLR2 and CX3CR1 genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs.

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