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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 14  |  Page No.: 2556 - 2563

Deep-Sequencing Analysis of DF-1 Cell Transcriptome Response to Infection with Newcastle Disease Virus of Duck

Shi-Min Gao, Min-Hua Sun, Zhao-Xiong Wang, Xian-Wei Li, Hui-Ying Fan and T. Ren    

Abstract: Newcastle Disease (ND) is caused by Newcastle Disease Virus (NDV) with a respiratory and digestive mucosal bleeding for typical symptoms. It is an acute highly contacting disease. NDV could multiplicate in DF-1 cells. NDV from waterfowl is different from former NDV from chicken, it lead waterfowl dead. But the molecular mechanisms of waterfowl NDV infected still are poorly understood. In this study researchers employed the illumina genome analyzer platform to perform genome-wide Digital Gene Expression (DGE), a tagbased high-throughput transcriptome sequencing method analysis of DF-1 cells infected NDV-YC. Researchers could reach a sequencing depth of 5.0-6.1 million tags per library and found >8000 genes to be differentially expressed during NDV-YC infection processes. NDV-YC notably identified genes involved in I-kappaB kinase/NF-kappaB and TGF-beta signaling pathway. Further analysis employed Gene Ontology (GO) analysis cell part, binding and biological regulation terms are main items, respectively. The data benefit for better understanding the molecular mechanism of NDV-YC infected and provide a basis for further in vivo study and clinical trials.

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