Comparative Study of Sedation, Pre-Anesthetic and Anti-Anxiety Effects of Hemp Seed Extract and Diazepam in Rats
Hempseed with scientific name Cannabis sativa, spreads across the world. Hempseed oils fatty acids compounds were obtained from different parts of Iran with different weather and were tested. The herb contains canabidole, canabinol and canabidiolic acid. The herb is used for preparing some sedation drugs which are used in neural stresses, anxiety, migraine, asthmatic coughs as well as local anesthesia in dentistry. Stuart points out that all parts of hempseed are used in China traditional medicine. Its seed is used as tonic, strengthening, laxative, diuretic, calmative, gastric anti parasite and narcotic. Masson and Teobold say that hempseed has a great value for tetanus treatment. Hempseed is a real calmative for stomachache and digestive disorders as well as beneficial for cancers, ulcers and rheumatic pains. In the present study, 30 Wistar male rats of 200-230 g weight and about 3 months old were used for laboratory experiments. Animals were kept in standard condition, at 20-25°C, 70% humidity and light cycle of 12 h lighting and 12 h darkness. Standard plates were used in order to feeding by method of ad libitum, i.e., 24 h feeding. Especial dishes were used for water. The rats were numbered in groups consisted of 5 animals and were placed in especial cages.