The Effects of Resveratrol Supplementation on Exercise-Induced Apoptosis
of Rat Hepatocytes
The present study was designed to determine the effects of resveratrol supplementation on exercise-induced apoptosis of rat hepatocytes. The rats were divided randomly into the sedentary group (Group I) and exhaustive exercise groups (Group I-IV). Each group contains 12 animals. The rats of exhaustive exercise groups received daily oral administration of resveratrol at doses of 0, 200 or 400 mg/kg/day for 30 days. The rats of sedentary group received daily oral administration of vehicle only (10 mL/kg/day) for the same period. Exhaustive exercise was performed on a motor-driven rodent treadmill. Paraffin-embedded liver sections were stained using the Streptavidin Peroxidase (SP) immunohistochemistry technique for BAX and Bcl-2 detection. The results showed that exhausting exercises could make the expression of BAX proteins in the liver and BAX/Bcl-2 ratio dramatically increases, the expression of Bcl-2 proteins obviously decreases. After resveratrol supplementation, the expression of BAX proteins and BAX/Bcl-2 ratio obviously decreased, the expression of Bcl-2 proteins obviously increased. Which indicated that resveratrol supplementation can inhibit exercise-induced apoptosis of rat hepatocytes.