Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 10  |  Page No.: 1757 - 1762

Optimization of Extraction Procedure for Chinese Truffle Tuber sinense Polysaccharides and Effect of the Polysaccharides on Phagocytosis of Macrophage in vivo

Ling Zhao, Kaiyu Wang, Xun Wang, Dan Huang, Juchun Lin, Defang Chen, Gang Ye and Cheng Lv    

Abstract: In this study, Response Surface Methodology (RSM) was employed to optimize the extraction conditions (extraction temperature, ratio of water to raw material and extraction time) of polysaccharides from the fruiting bodies of Chinese truffle Tuber sinense. A Box-Behnken design was used for experimental design and analysis of the results to obtain the optimal processing parameters. The optimal conditions were extraction temperature 94.75°C, ratio of water to raw material 16.41:1, extraction time 2.67 h. According to analysis, extraction temperature was the most significant factor to affect the yield of polysaccharides from Chinese truffle Tuber sinense. Pharmacological experiments indicated that CTP had potent immunomodulatory effects in immunosuppression mice model. At the dose of 50, 100 and 200 mg kg-1 body weight, a significant increase (p<0.01) in phagocytosis of macrophage was observed.

Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility