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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 7  |  Page No.: 1007 - 1011

Biochemical Differentiation between Bombina bombina bombina (Linneaus, 1761) and B. b. arifiyensis (Ozeti and Yilmaz, 1987) as Revealed by the Skeletal Muscle Protein Bands

Ufuk Bulbul, Sara Altinkaynak Eroglu, Ali Ihsan Eroglu and Zeynep Mutanoglu Kaya    

Abstract: In this study, skeletal muscle protein bands of the Bombina bombina specimens from five populations inhabiting in Turkish Thrace and Northwestern Anatolia were compared by SDS-PAGE (Sodium Dodecyl Sulphate-Poly Acrylamide Gel Electrophoresis). Totally 54 adult (26 females and 28 males) B. bombina specimens were used in SDS-PAGE characterization. B. b. bombina specimens in the Thrace group (Degirmenyeni, Buyukdoluk and Durusu populations) had 26 homologous protein bands whereas B. b. arifiyensis specimens in the Northwestern Anatolia group (Arifiye and Karasu) had 28 bands. It was shown that specimens of B. b. arifiyensis had two additional and unique bands. The results of the present study show that Thrace and Northwestern Anatolia specimens of the B. bombina populations in Turkey are different according to total number of skeletal muscle protein bands.

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