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Journal of Animal and Veterinary Advances
Year: 2011  |  Volume: 10  |  Issue: 18  |  Page No.: 2384 - 2388

In vitro Pharmacodynamics of Enrofloxacin Against an Escherichia coli gyrA Mutant

Murat Cengiz, Erdem Arslan and Ali Sorucu    

Abstract: The aim of this study was to investigate in vitro the pharmacodynamics of Enrofloxacin (ENR) against an Escherichia coli gyrA mutant (E. coli MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) amd multi-step resistance selection was performed until reaching 1 μg mL•1 MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for E. coli MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations • 8 times the MIC with no significant regrowth by 24 h.

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