Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Animal and Veterinary Advances
Year: 2011  |  Volume: 10  |  Issue: 18  |  Page No.: 2372 - 2375

Rescue of Duck Hepatitis Virus-1 from Cloned cDNA by Using T7 RNA Polymerase

M. Yu, Z.Y. Ning, Z.X. Cao, R.S. Li, C.L. Huang and G.H. Zhang    

Abstract: In the present study, researchers describe a rescue system for duck hepatitis virus-1 using T7 RNA polymerase. This strain can not induce typical cytopathic effect in BHK-21 cells. First, there is used the vitro transcripts to infect BHK-21 cells. Then the infected cell supernatant were inoculated into chicken embryos to rescue the virus and the cDNA clone was engineered to contain one silent nucleotide change to create a BssHII site to distinguish from the parental virus. The rescued virus was able to result in embryonic death and have similar infection with parental virus in chicken embryos indicating that the cDNA clones were replication competent. It is proposed that this rescue system may benefit the investigation on viral virulence determinants and molecular virology of DHV-1 virus.

Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility