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Journal of Animal and Veterinary Advances

Year: 2011  |  Volume: 10  |  Issue: 17  |  Page No.: 2234 - 2239

Populations of Salmonella enteritidis in Orally Infected White Chinese Goose

Guang-Zhi He, Yong Feng, Wei-Yi Tian, Ning Qian, Shu-Xuan Deng and Chuan-Wei An

Abstract

The objective of this study was to understand populations of Salmonella enteritidis (S. enteritidis) in the internal organs of geese after oral challenge. Researchers conducted serovar-specific Fluorescent Quantitative Real-Time PCR (FQ-PCR) for S. enteritidis to detect the genomic DNA of S. enteritidis in the blood and the internal organs including heart, liver, kidney, duodenum, jejunum, ileum, cecum, the bursa of fabricius, thymus, spleen and harderian gland from geese after oral challenge at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed. The results showed that S. enteritidis was consistently detected in all the samples. Ileum and jejunum were positive at 8 h PI and the last organ to show a positive result was the thymus at 36 h PI. The copy numbers of S. enteritidis DNA in each tissue reached a peak at 24 h and 2nd day PI with the blood, jejunum, ileum, liver and spleen containing higher concentrations than other tissues. However, the number of bacteria started decreasing by 2-3 days and by 6 days, the concentration of S. enteritidis DNA was below the detection limits of the FQ-PCR assay except the spleen. The IFA results were similar to those of the FQ-PCR. This study will help in understanding the pathogenesis of S. enteritidis infection in vivo.

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