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Journal of Animal and Veterinary Advances

Year: 2011  |  Volume: 10  |  Issue: 14  |  Page No.: 1863 - 1866

The Use of a Mixture of esat-6 and cfp-10 Protein as Antigens in ELISA for the Diagnosis of Bovine Tuberculosis in South China

X.D. Hu, K. Jia, G.H. Zhang, Z.X. Lin, H.Y. Xia, D.W. Zhang, X.B. Zeng, H.S. Qiao, H. Zhang and S.J. Li


Several Enzyme-Linked Immunosorbent Assay (ELISA) methods exist to diagnose Bovine Tuberculosis (BTB). In this study, an ELISA method was established with a mixture of esat-6 and cfp-10 as the diagnostic antigens. ELISA tests on 160 bovine serum samples (80 from cattle with BTB; 80 from healthy cattle) demonstrated that the sensitivity was 76.3% (61/80) and the specificity was 97.5% (78/80). A further screening test using ELISA and PPD was conducted with 335 serum samples collected at random from eight dairy herds (5 problematic herds and 3 BTB-negative herds) in Guangdong, China. In the problematic herds, the positive ELISA rate was 7.9% (16/202) for anti-esat-6 and cfp-10 antibodies and 5.4% (11/202) in the PPD. This study demonstrated that ELISA with the use of the esat-6 and cfp-10 mixture of antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of BTB.