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Journal of Animal and Veterinary Advances
Year: 2010  |  Volume: 9  |  Issue: 4  |  Page No.: 748 - 750

Introduction of Exogenous Gene into Chicken PGCs via Blastoderm

H. Furuta, T. Sanada, R. Takayanagi and T. Yoshida    

Abstract: It was proved that an exogenous gene was successfully transferred to chicken embryo and Primordial Germ Cells (PGCs) by the injection of the blastoderm. In this experiment, an exogenous gene, Lac Z constructs encoding Escherichia coli beta-galactosidase was introduced into chicken blastoderm (stage X) just ovoposition using lipofection reagent. After injection of gene the eggs were incubated in routine manner until developmental stage 12-15. Survival rate of the treated embryo was 35.6%. Lac Z expression was mosaic manner and low efficiency. However, it was obtained Lac Z specific band from chicken PGCs by PCR method.

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