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Journal of Animal and Veterinary Advances
Year: 2010  |  Volume: 9  |  Issue: 24  |  Page No.: 3117 - 3124

Resveratrol Inhibits Invasion and Metastasis of Hepatocellular Carcinoma Cells

Hai-Bo Yu, De-Yu Li, Hui-Feng Zhang, Huan-Zhou Xue, Cheng-En Pan, Si-Hai Zhao and Lei Wang    

Abstract: Resveratrol (trans-3, 4', 5-trihydroxystilbene) is a natural phytoalexin found in grapes and other plants that has anti-cancer and anti-inflammatory effects. To observe its effects of on proliferation of human hepatocellular carcinoma cells and the underlying molecular mechanisms, we performed the current study. MTT assay was used to assess the effects of resveratrol on proliferation of human hepatocellular carcinoma cell line HepG2. Flow cytometry and invasion assays were performed to detect apoptosis and tumor cell invasion capacity was detected in vitro and in vivo. After that Real-time quantitative PCR and western blotting assays were used to detect the expression of MMP-9 mRNA and protein, respectively. MMP-9 activity was also analyzed by gelatin zymography assay. NF-kappa B was measured by electrophoretic mobility shift assay. Resveratrol inhibited growth of HepG2 cells with a dose and time-dependent manner. The reduced cell viability is due to apoptosis or proliferation inhibited by resveratrol. Invasion assay found that resveratrol induce a decrease in the potential for invasion. Molecularly, resveratrol was able to reduce both MMP-9 mRNA expression and protein levels as wellas MMP-9 enzymatic activity which may be via the inhibition of NF-kappaB activity. The data showed that resveratrol was able to reduce viability of hepatocellular carcinoma cells which is due to induction of apoptosis by resveratrol. Resveratrol also inhibited tumor cell invasion capacity which is through down regulation of MMP-9 levels and its enzymatic activity. Future study will investigate the effects of resveratrol in vivo in control of hepatocellular carcinoma cell in clinics.

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