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Journal of Animal and Veterinary Advances

Year: 2010  |  Volume: 9  |  Issue: 2  |  Page No.: 284 - 286

Investigation of Prolactin Polymorphism in Buffalo Population of Khuzestan-Iran by PCR-RFLP

Y. Shojaei Tabar, J. Fayazi, H. Roshanfekr, Kh. Mirzadeh and A.S. Sadr

Abstract

Prolactin is a polypeptide hormone with multiple functions, secreted mainly by the anterior pituitary gland this experiment conducted to investigate of prolactin polymorphism in buffalo population in Khuzestan of Iran. DNA was isolated from blood samples of 85 buffalos of different areas. In this study, used Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique for amplified a 156 fragment consisting of part of exon 3. The amplified fragment digested with RsaI restriction endonuclease and subjected to electrophoretic separation in ethidium bromide-strained 3% agarose gel. All the samples studied comprised of single fragment of 156 bp indicating the monomorphic nature of the locus, showing AA genotype.

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