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Journal of Animal and Veterinary Advances

Year: 2010  |  Volume: 9  |  Issue: 2  |  Page No.: 281 - 283

Prolactin Genotyping of Najdi Cattle Breed Using PCR-RFLP

S. Sharifi, H. Roshanfekr, S.R. Khatami and K.H. Mirzadeh


Prolactin is a peptide hormone synthesized by lactotropes of the anterior pituitary. prolactin plays an important regulatory function in milk production and reproduction. Genomic DNA was isolated from blood samples of 84 Najdi cattle. A 156 bp PRL gene exon III segment was amplified by PCR using bovine specific primers. RFLPs in this segment was studied using RsaI restriction enzyme. The frequencies of genotypes were as follows: 0.2857-AA, 0.5714-AB, 0.1429-BB; Frequencies of allele A and B were 0.571 and 0.429 in Najdi Cows.