Effect of Single Nucleotide Polymorphism of IRF1 Gene on Cytokine Traits in Three Pig Breeds
In this study, IRF1 gene was chosen as a candidate gene to evaluate its effect on porcine cytokine traits in serum. A SNP in exon2 was demonstrated by sequencing and PCR-RFLP analysis. Cytokine traits include IFN-γ and IL10 concentrations in serum were measured when the pigs were at 20 and 35 days of age, respectively. The further association analysis between SNP genotype and cytokine traits were conducted in three pig breeds including Large White, Landraces and Songliao Black pig, one Chinese indigenous breed. The results indicated that the SNP of IRF1 gene had highly significant effect on level of IFN-γ (day 20; day 35) in serum (p = 0.0001; p = 0.0001) and ratio of IFN-γ to IL10 (day 35) in serum (p = 0.0083). The study suggested that the IRF1 gene could be regarded as a molecular marker gene for genetic selection of cytokine traits in the further disease resistance breeding.