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Journal of Animal and Veterinary Advances

Year: 2010  |  Volume: 9  |  Issue: 14  |  Page No.: 1902 - 1904

Detection of Allelic Polymorphism in a Gene of the Major Histocompatibility Complex of Iranian Buffalo

S. Rahimnahal, J. Fayazi, Kh. Mirzadeh, M.T. Beigi Nassiry and H. Roshanfekr


The Bovine Lymphocyte Antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. Since different alleles favor the binding of different peptides, DRB3 has been extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. Therefore in this study, the genetic diversity of the bovine class II DRB3 locus in the buffalo population in Khouzestan Province of Iran investigated by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method (PCR-RFLP). Buffalo DNA isolated from whole blood. A hemi-nested PCR followed by digestion with restriction endonucleases Hae III conducted on the DNA.