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Journal of Animal and Veterinary Advances

Year: 2009  |  Volume: 8  |  Issue: 5  |  Page No.: 935 - 945

Effects of Copper and Zinc on Cathepsin B Activity in Equine Articular Chondrocytes

Gustavo Hernandez Vidal, Jorge R. Kawas Garza, Rafael Ramirez Romero, Luis E. Rodriguez Tovar, Alicia M. Nevares Garza, Francisco A. Mora Valdez, Jesus J. Hernandez Escareno, Elisabeth Davis and Leo B. Jeffcott


The effects of copper and zinc on cathepsin B activity, chondrocytes behaviour and matrix turnover by articular cartilage were investigated in vitro using normal horse cartilage co-cultured with synovial tissue. Proteoglycan (PG) depletion was assessed by toluidine blue staining. Co-culture cartilage expressed elevated levels and activity of the PG-degrading enzyme cathepsin B. Copper but not zinc was able to reduce the depletion of PG and the enzyme activity induced by the synovial tissue.