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Journal of Animal and Veterinary Advances
Year: 2009  |  Volume: 8  |  Issue: 12  |  Page No.: 2429 - 2431

Effect of Different Protocol of Ovarian Stimulation, with Gonadotrophin on Concentration of Ovarian Hormones

F. Heidari, R. Sadrkhanlou, A. Rastegarnia and A. Ahmadi    

Abstract: The present study was undertaken to determine whether different protocol of gonadotropin, involved in regulation of ovarian steroidogenesis in female mice. The purpose of the present study was to determine the better time of treatment with gonadotropin. Thirty female mice in age from 4-5 weeks was used. All animal received 10 Iu gonadotropin (IP), then animals divided in 5 groups (n = 6 per group) group I = Control (primer injection), group 2 after 2 weeks, group 3 after 4 weeks group 4 after 6 weeks and group 5 after 8 weeks after primer injection super ovulated or stimulated by injection gonadotropin (10 Iu, IP). In each group, 46 h after injection, concentration of estrogen and progesterone were measured by Electrochemiluminescenc (ECL). Results showed that blood estrogen level decrease in group 2 and blood estrogen and progesterone. Concentration in group 3 was higher than other groups. The results of this study confirmed that the best time for treatment with gonadotrophin according ovarian hormones for IVF is 4 weeks after primer injection (group 1).

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