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Journal of Animal and Veterinary Advances
Year: 2009  |  Volume: 8  |  Issue: 8  |  Page No.: 1641 - 1645

Expression of Infectious Bovine Rhinotracheitis Virus Glycoprotein B in Bacterial Cell

Hassan Momtaz and Behnam Abbasian    

Abstract: Bovine Herpesvirus 1 (BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae, which contains three main gB, gC and gD genes and can cause different respiratory, reproductive and nervous system disorders in cows. In order to cloning of the coding region of gB gene of IBR virus, PCR product of the open reading frame of the gene from IBR-MDBK cell line was amplified by PCR. A 723 bp PCR product of the gB gene with EcoRI, SalI restriction sites were subcloned of pTZ57R/T and digested by the mentioned endonucleases. Digested insert cloned in to pGEX-4T-3 and transfected in E. coli cells. For the expression of gB protein, the pGEX-4T-3 recombinant vector was transformed and then induced in BL21 (DE3) strain of E. coli competent cells using IPTG, the presence of gB expressed protein was shown in immunoblotting and SDS-PAGE system. With respect to the remarkable frequency of infection to IBR in Iran and the necessity of controlling it through vaccination with recombinant vaccines of thymidine kinase, manufacturing and applying the recombinant gB protein are vital goals in recognition and distinction between infection and responses caused by vaccine.

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