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Journal of Animal and Veterinary Advances
Year: 2008  |  Volume: 7  |  Issue: 5  |  Page No.: 604 - 606

Determination of Hemagglutination Activity and Total Protein Recovered from Oil-Emulsion Avian Influenza Vaccines as a Prediction of Efficacy

Z. Rajabi, H. Tayefi-Nasrabadi, A.B. Syofi and H. Janmohammadi    

Abstract: The use of vaccination in poultry to control AI viruses, especially Mildly Pathogenic Avian Influenza (MPAI) viruses has been increasing in recent years. The amount of antigen mass and hemagglutination activity of Oil-Emulsion (OE) vaccines is important for the control of Avian Influenza (AI) viruses. With extraction of antigen mass of an AI OE-vaccine and determination of Hemagglutination (HA) titers and total protein; it is possible to predict the quality and protective efficacy of the vaccine. In this study, antigen mass was recovered from three commercially available AI OE-vaccines by aqueous partition method, then, the amount of extracted total protein and recovered HA activity were compared with the serologic responses caused in chickens by each vaccine. The results showed that the aqueous partition method retrieves antigen mass from the inactivated AI subgroup H9N2 OE-vaccines and also indicated that in three examined vaccines there is a good correlation among recovered HA activity, extracted total protein and antibody response in each vaccine. This study also showed that Determination of both recovered HA activity and extracted total protein let us know more about the efficacy of an OE-vaccine.

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