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Journal of Animal and Veterinary Advances
Year: 2007  |  Volume: 6  |  Issue: 2  |  Page No.: 152 - 154

Optimum Ficoll/Hypaque Gradient for Efficient Isolation of Peripheral Blood Monocytes from Camels

Layla Mohamed and M.M. Mukhtar    

Abstract: The viability, Purity and count of monocytes isolated from peripheral blood of camels were assessed after the isolation of these cells on 9, 10 and 12% concentrations of Ficoll/Hypaque. The results obtained showed that 97% viable cells were obtained using the three concentrations of the Ficoll/Hypaque. For purity, 12% Ficoll concentration separated cells were contaminated with others blood cells, while 9 and 10% separated cells were pure. When cells count was applied for the pure isolated cells, the cells isolated on 10% Ficoll/Hypaque concentration showed the best highest count compared to 9% with p = 0.003. Cells isolated using 10% Ficoll/Hypaque well proliferated when stimulated with Phytohaemaggglutnin (PHA) mitogen.

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