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Journal of Animal and Veterinary Advances
Year: 2005  |  Volume: 4  |  Issue: 8  |  Page No.: 706 - 707

Pulsed-field Gel Elecrophoresis for Comparison of Staphylococcus aureus SSP. Anaerobius Local Sudanese Isolates

Muna O. Elhaj and S.M. ElSanousi    

Abstract: Local Sudanese isolates of S. aureus ssp. anaerobius (which were isolated from sheep abscesses in the qurantene and slaughter house for exported sheep) were compared with the reference strain of S. aureus ssp. anaerobius on the genetic level by detection of the Restriction Fragment Length Polymorphism (RFLP) by Pulsed-Field Gel Electrophoresis (PFGE). PFGE involves embedding organisms in agarose, lysing the restriction endonucleases that cleave infrequently. Slices of agarose containing DNA fragments are inserted into the wells of an agarose gel and the restriction fragments are resolved into a pattern of discrete bands in the gel by an apparatus that switches the direction of current according to a predetermined pattern. The DNA restriction patterns of the isolates are then compared with one another to determine their relation. PFGE of S. aureus ssp. anaerobius local Sudanese isolates determined that all local isolates were identical, regarding the RFLP, whereas the reference strain have two bands at different locations, but it considered as same strain.

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