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Journal of Applied Sciences
Year: 2019  |  Volume: 19  |  Issue: 6  |  Page No.: 557 - 564

Production of Citric Acid from Corn Stalk through Submerged Fermentation Using Aspergillus niger

Judy Atabat Adudu, Shefiat O. Arekemase, Ibrahim Abdulwaliyu, Musa Latayo Batari, Helen Hoomsuk Raplong, Bamidele D. Aronimo and Yakubu Sani    

Abstract: Background and Objective: Many agro wastes have been explored for the production of citric acid. However, information on the use of corn stalk is scarcely available, hence, there is need to explore the use of corn stalk in the production of citric acid. This study is aimed at producing citric acid from corn stalk by submerged fermentation using Aspergillus niger isolated from the soil. Materials and Methods: Dried and pulverized corn stalk and A. niger obtained from soil samples were used in this study. The effects of carbon source, nitrogen source, pH, temperature, incubation time and methanol on citric acid production were studied. Results: Eight fungal isolates were characterized and isolate Asp 2 had the best citric acid production efficacy. Corn stalk powder mixed with 50% sucrose and 1 g of ammonium nitrate gave the highest yield of citric acid when compared to the other carbon and nitrogen sources. More so, pH 5, temperature of 30°C and 7th day of fermentation offered the highest yield of citric acid. Furthermore, a higher yield was obtained when 3% (v/v) methanol was added to the medium. Conclusion: Observation in this study deduced that corn stalk is a suitable and inexpensive substrate for the production of citric acid and could serve as value added agro wastes in the production of citric acid.

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