Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Applied Sciences
Year: 2019  |  Volume: 19  |  Issue: 3  |  Page No.: 217 - 222

Acute Toxicity Study and Determination of Median Lethal Dose of Catharanthus roseus in Wistar Albino Rats

M.G. Ajuru, G. Ajuru, F.W. Nmom, C.W. Worlu and P.G. Igoma    

Abstract: Background and Objective: Herbs were utilized by mankind since the ancient past, as alternative medicines for treatment of various diseases due to their presumed trustworthiness, efficacy, safety and low price. Catharanthus roseus (L.) G. Don is well known for its medicinal properties, like antibacterial, antifungal and antiviral. It is widely used in traditional medicine in India, Nigeria and West Indies to control diabetes mellitus. Toxicity studies serve as a preliminary study for identification of hazard and evaluation of the safety of any drug. This research was carried out to evaluate the acute toxic effects of ethanol leaves extract of C. roseus and to determine its median lethal dose (LD50). The objective was to investigate the toxicity of the leaves extract on the liver and biochemical parameters in rats. Materials and Methods: Single oral doses of the ethanol leaf extract of C. roseus was administered to albino rats at the concentrations 0, 1900, 3000 and 5000 mg kg–1. The animals were humanely sacrificed and blood and tissues obtained for biochemical and histological analysis on liver tissues. Results: The results revealed no significant changes in the control group but in the treatment group, there were changes such as dizziness, tremors and restlessness especially in treatment group D. Biochemical analysis showed normal alanine transaminase (ALT) levels in all treatment groups except in treatment group D where the level was significantly low (19 μ L–1). Aspartate transaminase (AST) was elevated in all treatment groups. Protein level was normal for all treatment groups except treatment group D where it was significantly low (26). Histological results showed normal liver tissues with hepatocytes, sinusoids and central veins. In treatment group B, there was tissue fragmentation with mild degeneration. In Group C, there was congestion in the central vein, vacuolation, fally, changes and necrosis of hepatocytes and connective tissues but in Group D, there were necrotic tissues in the liver. Conclusion: In this study, no mortality was recorded, therefore, the LD50 of the plant extract is slightly higher than 5000 mg kg–1 which can lead to mortality. It is recommended that studies on higher doses than the one in this study should be carried out to determine the actual LD50 of the drug.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility