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Journal of Applied Sciences
Year: 2015  |  Volume: 15  |  Issue: 11  |  Page No.: 1288 - 1296

Synthesis and Application of Glibenclamide Imprinted Polymer for Solid Phase Extraction in Serum Samples Using Itaconic Acid as Functional Monomer

Aliya Nur Hasanah, Rahmana Emran Kartasasmita and Slamet Ibrahim    

Abstract: Glibenclamide is a second-generation sulfonylurea drugs for treatment of diabetes mellitus. Up to now, a glibenclamide imprinted polymer is not reported for molecular recognition in biological samples. This research is conducted to have Molecular Imprinted Solid Phase Extraction (MISPE) for separation of glibenclamide from serum samples. The results showed that the itaconic acid is the functional monomer that provides the best interaction with the template (glibenclamide) from the computational study using Gaussian 09 software. The MISPE made from itaconic acid monomer at a ratio of 1:6:70 gives the best binding to glibenclamide in methanol pH 4. Serum sample which was spiked with glibenclamide gives recovery more than 80% after pretreatment with MISPE 2 in all concentration ranges. Selectivity test showed that MISPE 2 can be used for selective extraction of glibenclamide from serum samples spiked with other sulfonylurea drugs. This developed MISPE could be further used as extraction method in antidiabetic drugs analysis from biological samples.

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