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Journal of Applied Sciences

Year: 2014  |  Volume: 14  |  Issue: 12  |  Page No.: 1299 - 1303

Influence of Polymer Concentration on PVDF Membrane Fabrication for Immunoassay Analysis

A.L. Ahmad, N. Ideris, B.S. Ooi, S.C. Low and A. Ismail

Abstract

This study was aimed to study the influence of different PVDF membrane morphology on the adsorption of single proteins. Porous, symmetric PVDF membranes were prepared from PVDF/NMP solution by the phase inversion process. In this paper, the influence of polymer concentration in the 10.0-22.0 wt.% interval for the batchwise protein adsorption of BSA was investigated. The effect of polymer concentration on pore size distribution and surface morphology has been studied under capillary flow porometer and Scanning Electron Microscopy (SEM) respectively, which were further supported with the porosity test. All the synthesized membranes have a small range of pore size distribution with the decreased in pore size as the polymer concentration increased. It was found the membrane made of semi-crystalline PVDF showed a significant morphology change. For membrane performance, protein binding capacity showed the highest protein being retained at an optimum membrane formulation.

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