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Journal of Applied Sciences
Year: 2011  |  Volume: 11  |  Issue: 23  |  Page No.: 3797 - 3801

Mallow Carotenoids Determined by High-performance Liquid Chromatography

Jafar Muhammad El-Qudah, Jian Qin and Guangwen Tang    

Abstract: Mallow (Corchorus olitorius) is a green vegetable, which is widely consumed either fresh or dry by Middle East population. This study was carried out to determine the contents of major carotenoids quantitatively in mallow, by using a High Performance Liquid Chromatography (HPLC) equipped with a Bischoff C30 column. Neoxanthin and violaxanthin in mallow were characterized by Liquid Chromatography-Mass Spectrometry (LC-MS) with Atmospheric Pressure Chemical Ionization (APCI) as an ionization interphase. The total carotenoid content of mallow was estimated as 16.9 mg/100 g fresh weight. In which, major carotenoids in mallow are: lutein (5.3 mg/100 g), anhydrolutein (4.4 mg/100 g), β-carotene (3.7 mg/100 g), cis-anhydrolutein (1.0 mg/100 g), neoxanthin (1.6 mg/100 g), violaxanthin (0.3 mg/100 g), 9-cis-β- carotene (0.4 mg/100 g) and 13-cis-β-carotene (0.09 mg/100 g). Therefore, mallow is a rich source of carotenoids. As a popular vegetable consumed by the Middle East and surrounding populations, our finding of this study confirmed that mallow is an important source for carotenoids including provitamin A carotenoids.

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