Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Applied Sciences
Year: 2009  |  Volume: 9  |  Issue: 10  |  Page No.: 1907 - 1914

Development of a Polypyrrole-Based Amperometric Phosphate Biosensor

A.T. Lawal and S.B. Adeloju    

Abstract: A biosensor is developed by co-entrapment of Purin Nucleoside Phosphorylase (PNP) and xanthine oxidase (XOD) and potassium ferrocyanide (K4Fe(CN)6 into a polypyrrole (PPy) film via galvanostatic polymerisation of pyrrole. The optimum conditions for formation of the PPy-PNP-XOD-Fe(CN)64- film are 0.3 M pyrrole. 6.2 U mL-1 XOD, 49 U mL-1 PNP, 40 mM K4Fe (CN)6, polymerisation period of 200 sec and an applied current density of 0.5 mA cm-2. The optimum potential for the amperometric biosensing of phosphate was 200 mV vs Ag/AgCl (3 M KCl) in 0.05 M barbitone buffer. The achievable linear concentration range was between 0.1 and 1 mM, while the minimum detectable amount was 10 μM.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility