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Journal of Applied Sciences
Year: 2009  |  Volume: 9  |  Issue: 10  |  Page No.: 1907 - 1914

Development of a Polypyrrole-Based Amperometric Phosphate Biosensor

A.T. Lawal and S.B. Adeloju    

Abstract: A biosensor is developed by co-entrapment of Purin Nucleoside Phosphorylase (PNP) and xanthine oxidase (XOD) and potassium ferrocyanide (K4Fe(CN)6 into a polypyrrole (PPy) film via galvanostatic polymerisation of pyrrole. The optimum conditions for formation of the PPy-PNP-XOD-Fe(CN)64- film are 0.3 M pyrrole. 6.2 U mL-1 XOD, 49 U mL-1 PNP, 40 mM K4Fe (CN)6, polymerisation period of 200 sec and an applied current density of 0.5 mA cm-2. The optimum potential for the amperometric biosensing of phosphate was 200 mV vs Ag/AgCl (3 M KCl) in 0.05 M barbitone buffer. The achievable linear concentration range was between 0.1 and 1 mM, while the minimum detectable amount was 10 μM.

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