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Journal of Applied Sciences
Year: 2006  |  Volume: 6  |  Issue: 4  |  Page No.: 831 - 834

Extraction and Chromatographic Determination of Caffeine Contents in Commercial Beverages

Khalida Khan, Muhammad Naeem, Muhammad Javad Arshad and Muhammad Asif    

Abstract: High Pressure Liquid Chromatographic technique was employed to study caffeine contents in beverages. The samples were collected from local markets of Pakistan and determined its quality, quantity and suitability. A certified testing method for caffeine extraction was developed. Two extraction methods were used throughout this work solvent extraction and column extraction. The resulted extracts were concentrated then injected in HPLC equipped with ultra-violet detector. The quantitative results when compared with reference standard proved that column extraction is more selective then solvent extraction.

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