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Journal of Applied Sciences
Year: 2006  |  Volume: 6  |  Issue: 8  |  Page No.: 1745 - 1750

Polyhydroxyalkanoates Production by Recombinant Escherichia coli Harboring the Structural Genes of the Polyhydroxyalkanoate Synthases of Ralstonia eutropha and Pseudomonas aeruginosa Using Low Cost Substrate

Gustavo Graciano Fonseca and Regina Vasconcellos Antonio    

Abstract: Polyhydroxyalkanoates (PHAs) are thermoplastic, biodegradable polyesters, synthesized for some bacteria from renewable carbon sources. However, its application is limited by the high cost of production. To reduce these costs, recombinant strains that use diverse carbon sources have been developed. In this study, it was studied PHAs production by recombinant Escherichia coli (DH10B and JM10), harboring the structural genes of the polyhydroxyalkanoate synthases of Ralstonia eutropha and Pseudomonas aeruginosa, using hydrolyzed corn starch and soybean oil as substrate, cheese whey as supplement and acrylic acid as fatty acids β-oxidation inhibitor. The effect of these on the cell mass and the PHA content had been evaluated through an experimental design 24. The best results had been obtained with DH10B strain, with a dry cell weight of 0.92 g L-1 and 5.9% of PHA (0.5 mol% 3-hydroxybutyrate, 44.9 mol% 3-hydroxyhexanoate and 54.6 mol% 3-hydroxyoctanoate), in mineral media containing 5% of soybean oil, beyond 1 mmol of acrylic acid.

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