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International Journal of Zoological Research
Year: 2017  |  Volume: 13  |  Issue: 1  |  Page No.: 6 - 11

Detection of Toxoplasma gondii from Wastage Nourished Small Ruminant and Poultry: Zoonotic Significance

Hassan A. Elfadaly, Mohey A. Hassanain, Raafat M. Shaapan, Nawal A. Hassanain and Ashraaf M. Barakat    

Abstract: Background: The wastages nourished small ruminants and poultry are still free fed on street wastages and possibly exposed to T. gondii oocysts through feces of outdoor shedder cats and they are regarded as high prevalent sources for human toxoplasmosis via their meat containing viable T. gondii tissue cysts. Materials and Methods: A total No. of 859 samples of both blood and their matching tissue were collected from wastages nourished 455 sheep, 237 goats, 124 chickens and 43 ducks respectively from Giza governorate, Egypt. All animals were assayed serologically using Latex Agglutination Test (LAT) as a screen test and the results were confirmed by ELISA. Tissue samples which were identical to seropositive sera were digested and microscopically examined and exposed to DNA confirmation. The microscopic definite bradyzoites containing sera were bio-assayed through intra-peritoneal passage in mice as viability test to determine both LD50 and LD100 for each species isolate. Histopathological examination was done on symptomatic morbid and dead mice. Results: Corresponding to small ruminants and poultry, results of seropositive percentages were 47.5 and 29.3%, total microscopic 30.1 and 32.7%, DNA detection 74.8 and 71.4% and the total percentages of mice viability test 39.4 and 31.3%. In Addition, the total percentages of LD50 were 30.3 and 31.3%, while the LD100 were 9.1 and 0% in small ruminants and poultry respectively. The histopathological examination of inoculated mice signified cyst forming T. gondii in acute and chronic lesions within vital organs. Conclusion: The wastages nourished small ruminants and poultry is of zoonotic impact and significance and must be directed for incriminate this animal feeding pattern and for avoiding consumption under cooked meat of animals or birds.

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