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International Journal of Zoological Research
Year: 2008  |  Volume: 4  |  Issue: 1  |  Page No.: 77 - 80

Effect of Storage Period on the Efficacy of African Bull Frog Pituitary Extract for Induced Spawning of Clarias gariepinus

O.T. Adebayo and O.A. Fatoyinbo    

Abstract: Acetone dried pituitary extract obtained from African bullfrog, Rana adspersa, was stored over a period of 16 weeks and used to induce spawning in the female Clarias gariepinus. Fresh pituitary extract was used as control. Fecundity and egg weight decreased with storage period. Fish injected with pituitary extract stored for 12 weeks and above had significantly (p<0.05) lower % fertilization and % hatching compared with fish stored for less than 4 weeks. The potency and viability of R. adspersa pituitary extracts decreased with storage periods. The results of this study indicated that stored R. adspersa pituitary hormone extract stored for 4 weeks gave the best result. Its yield was significantly different from that gotten using hormones stored for over 8 to 16 weeks period. It showed that the frog pituitary extract is still potent, viable and effective when stored for not more than 4 weeks. The R. adspersa pituitary hormone stored for 4 weeks can therefore be used as optimum storage period under the experimental conditions used in this study.

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