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International Journal of Virology
Year: 2017  |  Volume: 13  |  Issue: 1  |  Page No.: 53 - 61

Characterization of Citrus tristeza virus (CTV) Isolated from Dakahlia Governorate, Egypt

Adel A. El-Morsi, Samia A. Haroun, Ayat M. Hassan, Dalia G. Aseel and Elsayed E. Hafez    

Abstract: Background and Objective: Citrus tristeza virus (CTV) is the most destructive virus of citrus which resulted in huge economic losses to citrus crop in Egypt and all over the world. We aim in this study to determine the presence, distribution and well characterize the virus in Egypt. Materials and Methods: A number of 77 naturally infected leaves and leaf petioles of citrus plants were collected from different cultivated areas in Dakahlia governorate, Egypt. The symptoms in infected plants included tristeza or quick decline, vein clearing and leaf cupping. Moreover, stem pitting of the trunk and branches of infected trees was observed. The leaves were collected and subjected to ELISA test. Results: The results revealed that about 84% of the collected samples with symptoms were positive. The ultra thin sections of CTV-infected leaves showed the presence of viral arrays, fibrous inclusions and accumulated cytoplasmic vesicles. Also, masses of electron-dense bodies were found between the cell wall and cell membrane. Accordingly, lipid accumulations and multi vesicular bodies were abundant in the cytoplasm. More effects were observed such as nuclear membrane invaginations and chloroplast degradation. Electron microscopy examination for the purified preparation (based on PEG precipitation and differential centrifugation) revealed the presence of filamentous virus-like particles with size about 2000×11 nm. Conclusion: The virus characterization has a vital role for rapid detection of CTV and prevents prevalence to other areas. The study proved the presence of tristeza virus in Egypt and the stockholder should take steps to control its distribution all over Egypt. The virus completely destroys the cell and it is highly distributed in all visited areas. Further studies should be carried out to find suitable tools for preventing the virus hassles for citrus production.

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