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International Journal of Virology

Year: 2014  |  Volume: 10  |  Issue: 3  |  Page No.: 224 - 234

Detection of Three Potato Viruses Isolated from Saudi Arabia

M.S. Al-Saikhan, K.A. Alhudaib and A.M. Soliman

Abstract

Potato Leaf Roll Virus (PLRV), Potato Virus Y (PVY) and Potato Virus X (PVX) are the most economically important viruses in commercial potato production. Enzyme Linked Immunosorbent Assay (ELISA) and the Reverse Transcription Polymerase Chain Reaction (RTPCR) were used for the detection of these three potato viruses. Specific primer pairs designed to amplify the coat protein gene of each virus (627 bp for PLRV, 801 bp for PVY and 714 bp for PVX) were successfully applied. A multiplex RT-PCR (mRT-PCR) was developed for the simultaneous detection of the three viruses in potato leaves. The nucleotide sequences of the coat protein genes of the Saudi isolate of PLRV (PLRV SA3), the Saudi isolate of PVY (PVY SA2) and the Saudi isolate of PVX (PVX SA1) were submitted in the GenBank under accession numbers: KC875235, KC875237 and KC875236, respectively. The nucleotide sequences PLRV SA3, PVY SA2 and PVX SA1 were compared to the sequences of the coat protein genes of other PLRV, PVY and PVX isolates. The similarity of the nucleotide sequences suggested that the architecture of the polerovirus (PLRV), potyvirus (PVY) and potexvirus (PVX) are highly conserved. This study describes an assay where three common potato-infecting viruses, Potato leafroll virus, Potato virus Y and Potato virus X, were detected simultaneously from total RNA potato leaves in a multiplex RT-PCR.

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