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International Journal of Virology
Year: 2012  |  Volume: 8  |  Issue: 3  |  Page No.: 240 - 257

A New Egyptian Satellite Strain of Cucumber Mosaic Cucumovirus

A.A. Megahed, Kh. A. El- Dougdoug, B.A. Othman, S.M. Lashin, M.A. Ibrahim and A.R. Sofy    

Abstract: Cucumber mosaic cucumovirus satellite strain Egyption isolate (st-CMV-EG) was detected using polyclonal antibodies by DAS-ELISA form naturally infected cucumber leaves collected from protected agriculture. The samples which give positive reaction were used to virus isolation. st-CMV-EG isolate has a wide host-range belonging to 6 families. The virus isolate was inactivated at 70°C, it was infected at dilution 10-4 and longevity was 4 days at room temperature. Amorphous and crystalline inclusion bodies were detected in hair cells of epidermal strips of CMV-inoculated cucumber leaves. The U.V absorption ratio, A260/A280 and Amax./Amin. were 1.204 and 1.101, respectively, as well as virus yield was 1.43 mg/100 g fresh leaves in purified virus preparation. Electron micrograph of the purified virus isolate showed spherical particles (28 nm.). The virus isolate was detected serologically by using dot blot immunoassay. CMV-RNA was successfully detected in infected cucumber leaves using specific primer of cp gene by RT-PCR and the expected size was about 582 bp. Sequence analysis of CMV-cp gene of cucumber Egyption isolate was indicated 38% similarity to that of AB024493 and D00542.

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